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Sample preparation and quantification for d9-carnitine, d9-TMAO, and d9--butyrobetaine of humanized gnotobiotic mice model Twenty microliters of plasma and 10 L of urine samples were extracted with 180 L and 190 L methanol containing 50 ng/mL isotopically labeled internal standards (d3-carnitine and 13 C 3 -TMAO) respectively, and then vortex for 3 min

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Model: Middle-aged (10- to 12-month-old) male C57BL/6 mice Dosage: Intraperitoneal injection of 5 nmol/kg/day of the GLP-1 receptor agonist exenatide for 30 weeks