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NAC was prepared in phosphate-buffered saline the day prior, balanced for pH (using NaOH), stored under nitrogen, and opened immediately prior to each injection to minimize oxidation

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This transfer is crucial for mitochondrial quality control, enabling recipient cells to replenish damaged mitochondria and degrade dysfunctional ones (Fig

Both are shown in Figure 1 as part of a redox tower of key biological half reactions that can be linked to find the overall redox potential change and thus the free energy