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Inhibition of 5-LOX in a Cell-Free Assay The enzyme activity of human recombinant 5-LOX was determined spectrophotometrically by monitoring the oxidation of H 2 DCFDA to the highly fluorescent 2,7-dichlorofluorescein during the catalytic reaction mediated by 5-LOX as reported ( Inhibition of LTA 4 H in a Cell-Free Assay Leukotriene A4 hydrolase activity was measured by monitoring the formation of LTB 4 with an enzyme-linked immunosorbent assay