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Landmark roughly two to three finger-widths below the acromion process, into the densest part of the muscle, well clear of the shoulder bursa

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Ultrapure water (18.2 M cm resistivity, Arium Comfort H2O-I-1-UV-T from Sartorius, Goettingen, Germany) or LC-MS grade water (LiChrosolv, Merck KGaA) was used throughout

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