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Reports have shown that 90% of curcumin degradation under alkaline and neutral conditions occurred within 1530 min, while in acidic incubations, degradation was substantially slower, with less than 20% of total curcumin degraded within 60 min (71, 92)

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Second staining Subsequently, sections previously stained for S100, MAOB, and ABAT were incubated with guinea pig anti-GABA (1:100, Millipore ab175) and chicken anti-GFAP (1:200, Abcam ab4647) antibodies for 24 h

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