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The homogenates were then centrifuged for 40 min at 350,000 g in 500 L polycarbonate centrifuge tubes (Beckman Coulter) at 4 C in an Optima MAX-XP Ultracentrifuge with a TLA 120.1 rotor (Beckman Coulter)
The same body of research also found that higher doses of GHK-Cu can produce skin irritation, and that copper in general is highly toxic, which is why formulation and concentration matter considerably in any experimental design involving this compound
133 Solid tumor staining is consistent with this perspective
Allen and R
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