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H., Preckel, B., & Hermanides, J
If your vial contains overfill and you calculate based on labeled mass, your final concentration will be proportionally higher for example, a 5mg vial with 5.4mg actual content reconstituted in 0.5mL yields 10.8mg/mL instead of 10mg/mL, an 8% difference that is generally acceptable within research tolerances of plus-or-minus 10%.
When MTHFR diverts 5,10-MTHF to 5-MTHF production, less 5,10-MTHF is available for TYMS reducing dTMP synthesis and causing the intracellular dUTP/dTTP ratio to shift toward excess dUTP
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