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Before each assay appropriate amount of aliquots were thawed, suspended (1 10 5 cells/ml) in assay medium (DMEM w/o phenol red, 10 mM Hepes, 2% ovalbumin and 0.2% Pluronic F-68) and then transferred into a 96 well plate (5 10 3 cells/50 l) followed by addition of ligand and/or Fab 3F52 diluted in assay medium (50 l/well)

By 24 hours post-injection, greater than 95% of the previous dose has cleared from circulation, making daily administration feasible for sustained research protocols without reaching toxic plasma concentrations

The medication may have lost some potency

All participants completed mRNA vaccination, while the intervention started ten days after the first dose

Those with a stressful lifestyle and/or profession