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Three-week old, female Swiss-Webster mice obtained from the Charles River were housed in the Comparative Research Center of Rush University Medical Center
Approximately 110 L of working solution containing 10 L of cell counting Kit-8 (YOBIBIO, Shanghai, China, #U22-001A) and 100 L of DMEM/F-12 were added into each well of 96-well plates
The fine-gauge, short-needle design supports subcutaneous injection with minimal discomfort
It is important to acknowledge the limitations of this study
Our hypothesis is that deficient eating habits, autoimmune regulator gene predisposing gene, dysbiosis and molecular mimicry, unfolded proteins and stress in the endoplasmic reticulum, and thymus involution appear to be the main potential factors leading to HT oxidative stress
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