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Th1 chemokine interferon inducible protein 10 (IP-10) levels have been shown to be elevated in BAF of asthmatic children, and apigenin also reduced its production by monocytes, coinciding with inhibited phosphorylation of JNK and MAPK pathways [140]

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Size-selected cDNA libraries were amplified using indexed primers for 1214 cycles in a 50 l PCR containing 29 l of template, 1 l of forward and reverse primers (25 M each), and 1 U of Phusion high-fidelity DNA polymerase (New England Biolabs)

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