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Specifically, IF markedly increased the expression of glutathione peroxidase 4 (Gpx4) and heat shock protein 1 (Hspb1), while inhibiting the elevation of ferroptosis-promoting factors such as Nfe2l2, Slc7a11, and Alox8

Gradients were centrifuged at 18,000 rpm in an SW-28 ultracentrifuge swinging bucket rotor (Beckman/Coulter) for 20 min at 4 C to separate host cells debris

Bepirovirsen, developed by GlaxoSmithKline (GSK), is a 2-MOE-modified ASO that targets all HBV RNAs, promoting RNase H-mediated RNA degradation, reducing HBV replication, and inhibiting HBsAg production