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Further, nitrogen fixation related genes nifD1 (K02586), nifH (K02588), and nifK (K02591) were present in all moist STPs and the abundance of nifD1 and nifK were high in Q3, Q1, MS2, Q2, and K1 whereas nifH was prevalent in Q3, K2, K3, Q1, MS2, Q2, and K1 (Figure 6 and Supplementary Table 6)

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Methylation is an important epigenetic modification, which modifies the methyl groups in gene without changing gene sequence of bases 49