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RNA strand has internal promoters used by replicase to make mRNA for 30K protein (MP) and 17.5 K (CP) promoters Transcription by RdRp MP mRNA CP mRNA TMV Life cycle (cont.) h) MP combines with viral +RNA to move it into new plant cells through plasmodesmata TMV Life cycle (cont.) i) Accumulation of +RNA & CP proteins stimulates assembly of progeny virions Viral RNA interacts with discs CP units self assemble into discs Electrostatic interaction stabilizes structure into helix M