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Materials and Methods RAW264 Cells, GSH Depletion, and Treatment RAW264 cells were cultured in Roswell Park Memorial Institute medium 1640 with 2 mM l-glutamine (Sigma), 100 U/ml penicillin, 100 g/ml streptomycin sulfate (Invitrogen/Life Technologies), and 10% heat-inactivated FCS (Sigma)-omplete medium

Sterile water has no antimicrobial preservative

A high-protein diet and physical activity will help enhance the effectiveness of these supplements and achieve better results

Most people see the best results with a series of treatments spaced a few weeks apart, usually between 4 to 8 sessions