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Studies about these five autophagy receptors were knocked out by CRISPR-Cas9 in cells indicated that only NDP52 and OPTN are primarily essential for mitophagy, which may be due to the function of NDP52 and OPTN in growing the isolation membrane to amplify mitophagy through an NDP52/OPTN-Ulk1-ATG8-NDP52/OPTN-dependent positive feedback loop, as NDP52 and OPTN have lower binding affinity to all ATG8 family proteins compared to P62 and NBR1

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