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The V3 to V4 region of the 16S rRNA gene was amplified via polymerase chain reaction (PCR) employing the 341 F and 805R primers, while the fungal internal transcribed spacer (ITS) region was amplified using the ITS-1F and ITS-1R primers
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The report observed: "the possible adverse physiological effects of elevated levels of these medications cannot be discounted, especially in her weakened state." [117] On December 24, 2009, Murphy was buried at Forest Lawn Memorial Park in Hollywood Hills
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