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doi: 10.1016/j.lfs.2021.120238 [DOI] [PMC free article] [PubMed] [Google Scholar] 20.Fletcher NM, Abusamaan MS, Memaj I, et al
ALT levels dropped from 68.9 to 58.1 IU/L that's significant because liftd ALT is a key marker of liver cell damage
Depletion of intracellular GSH is a hallmark of ferroptosis, as it impairs antioxidant defenses
Briefly, cells plated at a density of 2 10 5 cells/cm 2 in 12-well tissue culture-treated polystyrene plates, were first grown for 2 weeks in Williams E medium (Invitrogen, Cergy-Pontoise, France) supplemented with 10% (vol/vol) fetal calf serum (FCS) (Cytiva, Velizy-Villacoublay, France), 100 IU/ml penicillin, 100 g/ml streptomycin, 5 g/ml bovine insulin (SigmaAldrich, Saint-Quentin-Fallavier, France), 2 mM glutamine (Invitrogen) and 50 M hydrocortisone hemisuccinate (Upjohn, Paris La Dfense, France)
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