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In order to inhibit endogenous peroxidase activity slides were incubated in 0.3% perhydrol (H2O2) in methanol for 30 min

However, even after iodization, the odds ratio for having thyroid enlargement was increased for smokers and smokers still had a lower mean thyroid stimulating hormone and a higher free thyroxine in serum than nonsmokers.[] The two most common hypotheses to explain this phenomenon involve the role of thiocyanate and thyroglobulin

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In addition, traditional hierarchies of evidence that value randomized controlled trials and laboratory experiments above other types of evidence generally do not work for research on the social determinants of health