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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

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Furthermore, this damage causes a mutation in the DNA molecules regardless of their location, which eventually causes defects in the translated proteins and ultimately impacts multiple facets of cellular physiology [121]

(iii) resveratrol (Res) group, microglia (~ 100,000 cells/ml) cultured in complete medium containing the Sirt1 agonist resveratrol (20 mol/l

(2020, April 1)