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Tobacco expressing OsHMA3 under the control of a root-specific promoter from a tobacco root extension-like protein-coding gene ( NtREL1 ) resulted in the root-specific retention of cadmium, consequently lower root-to-shoot translocation and decreased accumulation in the shoots was generated (Cai et al

The sample with the eluted fraction containing the unprocessed form run for 15 min and the sample with purified cardosin B was run until the three bands were clearly separated