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2.7 G protein activation assay by Nb37 BRET A construct encoding SNAP-GLP-1R with a C-terminal nanoluciferase was generated in house by PCR cloning of the nanoLuciferase sequence from pcDNA3.1-ccdB-Nanoluc (a gift from Mikko Taipale

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Under physiological conditions, the kinase PINK1 is stabilized on the outer membrane of depolarized mitochondria, subsequently recruiting and activating the E3 ubiquitin ligase Parkin to flag the damaged organelle for autophagic clearance