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Simple absence is defined as very brief clouding of the sensorium or loss of consciousness accompanied by certain generalized epileptic discharges without other detectable clinical signs

Sample preparation (B) In an alternate protocol, the frozen liver tissue was directly lysed by probe sonication (2 10 pulse, 40% power output) in 4 M urea/DPBS and briefly cleared of debris by centrifugation (5000 g , 5 min, 4 C)