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Key nodes include: 3.1.1 GLUT1/3: mediated glucose uptake Glucose transporter1 (GLUT1) expression is modest in resting T cells, but co-stimulation through T cell receptor (TCR) and CD28 quickly triggers the phosphoinositide 3-kinase (PI3K)/Protein Kinase B (Akt)/mTOR signaling cascade, which results in GLUT1 transcriptional upregulation and membrane translocation ( 3.1.2 PKM2: driven metabolic rewiring Pyruvate kinase is the rate-limiting enzyme that catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, serves as a critical metabolic and immunological node in T cell fate determination

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This limits exposure to the treatment site and reduces potential for systemic side effects

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