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In cognitive peptides: substitute when possible in vivo tests by cerebral organoids or primary neuronal cultures ( Replacement ), optimise experimental plan to minimise the number of animals ( Reduction ), use appropriate anaesthesia and analgesia during invasive procedures ( Refinement )

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by: 26 Sikiric, P., Seiwerth, S., Rucman, R., Turkovic, B., Stancic Rokotov, D., Brcic, L., Sever, M., Klicek, R., Radic, B., Drmic, D., Ilic, S., Kolenc, D., Vrcic, Hrvoje., & Sebecic, B