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In addition, tobacco product regulation became a global issue with development and implementation of the 2005 World Health Organization (WHO) Framework Convention on Tobacco Control (FCTC) [11], whose articles 9, 10, and 11 require parties to regulate tobacco products

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Looking forward, strategies that decouple donor availability from prolonged effector expression, such as RNA virus-mediated delivery, inducible or transient effector expression or fully RNA-based R2 systems, represent promising routes to mitigate rDNA perturbation while preserving integration potential

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