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The viral titer of concentrated viruses was calculated using the qPCR Lentivirus Titration Kit from ABM (Applied Biological Materials, Vancouver, Canada) according to the manufacturers instructions
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In vitro, high copper concentrations (0.40.6mM) have been shown to activate NFKB in HepG2 cells [24], and this activation appears crucial for cell survival under copper stress [26]
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Copper tripeptide-1 is by far the most potent antiaging copper compound
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