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Moreover, this signature panel showed promising results in predicting MCI progression to AD after monitoring patients for 1 to 11 years (Siedlecki-Wullich et al., 2019)

(4) Add 300 L of chloroform-methanol (V:V=2:1) into the centrifugal tube with the lower layer of solution, vortex for 30 s, and ultrasonic extraction for 10 min in an ice-water bath