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This gentle agitation is key to maintaining the peptide's molecular structure

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The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the antibody (R1401-12) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system

JSLS : Journal of the Society of Laparoendoscopic Surgeons

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