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For cell sorting of Lenti-X 293T, cells were rinsed twice with 500 L PBS and then treated with 300 L Non-enzymatic Cell Dissociation Solution (13151014, Gibco) for 46 min at 37C to detach cells

Since the biosynthetic pathways for many sulfur-containing natural compounds include Mca homologs, the involvement of LMW thiols in natural product biosynthesis might be a common mechanism (Zhao et al., 2015)