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IVM medium applied in this study was TCM-199 medium supplemented with 0.3 mM sodium pyruvate, 1 g/ml -2-oestradiol, 2 mM Gluta MAX , 10 ng/ml EGF, 10% fetal bovine serum, 10 U/ml FSH, 10 U/ml LH and 100 M cysteamine (6769)

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