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Preparation of cigarette smoke extract CSE was prepared by combusting one standard filtered cigarette and channeling the smoke through 10 mL of PBS via a vacuum pump and a custom syringe-based apparatus to produce 100% CSE

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Briefly, the epidermis collected by suction blister was homogenized and the total RNA was isolated

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Yes, vaping can raise blood sugar levels due to the nicotine in e-cigarettes, which stimulates glucose release from the liver