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These mechanisms lead to the prolonged activation of trigeminal neurons, astrocytes, and microglia around meningeal blood vessels, inflammatory cascades within brain parenchyma reaching the glia limitans, and the dispersal of arachidonic acid (AA) and potassium ions into the perivascular area (9, 10, 39)

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To construct the sorting vector for gene overexpression, we amplified the whole expression cassette of N-signal-GST-EGFP-GPI DAF and N-signal-GST-EGFP-GPI CEAM7 from pEGFP-GST-GPI DAF and pEGFP-GST-GPI CEAM7 vectors and joined them with PCR linearized pcDNA3.1 vector using ClonExpress II One Step Cloning Kit

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Enzymes may degrade surface coatings, impacting circulation time and biocompatibility [75]