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An increase in ROS production also occurs during differentiation due to the dramatic increase of NADPH oxidases activity [48]

**Chitosan (derived from crustaceans)

This cascade results in irreversible proteotoxic stress, ultimately leading to cardiomyocyte death [74]

A systematic rotation pattern (for example, moving clockwise around the abdomen, or alternating between left and right thigh) helps minimise this risk