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As a major cell type in kidney, proximal tubular cell produce ATP via fatty acid -oxidation (FAO) in mitochondria ( via controlling the transcription and translation of FAO-related enzymes (Xu et al., 2022), which could further trigger the impairment of mitochondrial structure and function ( 3 Natural products for regulating mitochondrial function 3.1 Enhancing mitochondrial biogenesis and energetics It is widely found that impaired mitochondrial biogenesis and energetics contribute to the pathogenesis of kidney diseases (Weinberg, 2011

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So the similarity in the constraints of 1-Cys and 2-Cys reduction facilitates this second Cys acquisition step, but an additional constraint is that the second Cys must be the resolving Cys and thus have a higher pKa than the existing Cys

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