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Continuous use without breaks can reduce receptor sensitivity and potentially cause copper dysregulation

After derivatization, the samples were transferred into GCMS vials and analyzed using GCMS (Shimadzu GCMS-QP2010 Ultra) with a DB-5MS stationary phase column (30 m + 10 m DuraGuard 0.25 mm i.d

Am J Hypertens 27(1):130139 Kim M, Platt MJ, Shibasaki T, Quaggin SE, Backx PH, Seino S, Simpson JA, Drucker DJ (2013) GLP-1 receptor activation and Epac2 link atrial natriuretic peptide secretion to control of blood pressure

Acsl4 fl/fl Ksp Cre-ERT2 mice and Fsp1 fl/fl Ksp Cre-ERT2 mice underwent tamoxifen-inducible recombination validation via PCR genotyping and subsequent Western blot confirmation of target protein ablation

Louis, Mo, USA) supplemented with 10% iron-fortified bovine calf serum (SAFC Biosciences, Lenexa, Kan, USA), 2 mM l-glutamine (Gibco, Carlsbad, Calif, USA), and antibiotic/antimycotic (100 U/mL penicillin, 100 g/mL streptomycin, and 0.25 g/mL amphotericin B