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Most protocols suggest consistency in timing, same day and approximate time each week

In the UK, typical oral doses for dietary deficiency are 50-150 micrograms daily, with higher doses (1000-2000 micrograms) generally reserved for clinical situations under healthcare supervision

Quantitative Real-Time PCR A total RNA extraction kit (Vazyme, Nanjing, China) was used to collect total RNA from TPC-1 and KTC-1 cells

stored all gathered samples in facilities where they could be kept permanently and made available to other scientists, hence reducing the requirement for additional collections