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IGF-1 DES was originally researched for medical uses, including: Muscle wasting diseases Cachexia Injury rehabilitation Recovery from surgery Support in aging-related muscle loss The goal was to harness IGF-1s anabolic and regenerative potential while reducing systemic effects and controlling the location of its action

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Using a robotic liquid handling system (Tecan Freedom EvoR), 200 l of the cell culture was dispensed using the LIH arm, into six sterile 96 well plates sequentially, except for the first and last columns which were reserved for no compound controls