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Genetic basis for increased intestinal permeability in families with Crohn's disease: role of CARD15 3020insC mutation

In our study, despite lower raw quality scores, we achieved complete coverage of target regions and 100% concordance in variant detection relative to Illumina-based results, confirming its accuracy and reliability, hence demonstrating that the lower per-base quality does not compromise diagnostic accuracy

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Fitzgerald R, Bass LM, Goldberg DJ, Graivier MH, Lorenc ZP