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S-Adenosyl methionine quantification assay Intracellular SAM levels were quantified with Bridge-It S-Adenosyl Methionine (SAM) Fluorescence Assay (#1-1-1003, Mediomics)

Elevated GGT degrades glutathione (GSH), releasing harmful hydrogen sulfide and reducing antioxidant and mitochondrial activity 63 64 35

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Another major advancement is the application of omics-based technologies such as metabolomics, gut microbiome profiling, and transcriptomics to understand the mechanisms of herbal action and host interaction