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Membranes were probed with primary antibodies (all at 1:1,000 dilution) overnight at 4 C, and subsequently incubated with IRDye 800CW-conjugated goat anti-rabbit IgG or goat anti-mouse IgG (both at 1:10,000 dilution

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Best practices: Store reconstituted blends at 2-8C, use within 2-4 weeks, protect from light and oxygen, and discard if you observe color changes or precipitation.

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Learning and caring in communities of practice: using relationships and collective learning to improve primary care for patients with multimorbidity