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The GHK sequence also appears within the alpha-2(I) chain of type I collagen, leading to the hypothesis that collagen degradation at wound sites releases GHK fragments that then bind copper and signal for new collagen production, creating a self-repair feedback loop

Sections were fixed in ice-cold 0.5% glutaraldehyde (w/v, PBS) for 15 min and washed with 1 mM MgCl 2 /PBS (pH 6.0) for 5 min

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References Akmal, U