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Cellular-level changes IGF-1 levels, inflammatory markers, telomere metrics are tracked through labs at the end of each 12-week cycle

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Determination of Michaelis-Menten constant for monobromobimane For validation of the assay, the enzymatic reaction between monobromobimane and glutathione was characterised to determine kinetic parameters such as apparent Michaelis-Menten constant and maximum rate of the reaction