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injections of 100 nmol kg 1 GLP-1 ( n = 6 mice), 100 nmol kg 1 GLP-1MK-801 (Pen-linked) ( n = 6 mice) or vehicle (isotonic saline, n = 6 mice) for 7 days

Cytotoxicity in mammalian cells Vero cells (African Green Monkey Kidney cells, ATCC CCL-81) were grown in Dulbeccos modified Eagles medium (DMEM) containing glucose, L-glutamine and sodium pyruvate and supplemented with 10% fetal bovine serum and 1X penicillinstreptomycin (Sigma) at 37 C in culture flasks (TPT-90025) until confluent growth was attained

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Miguez-Pacheco, V., Hench, L

Since the body cannot synthesize sufficient amounts of vitamins, it is necessary to take them through food or supplements