The binding of HGF to c-met was detected with biotinylated anti-HGF polyclonal Ab (1:500 dilution in the 0.1% BSA-TTBS for 3 h at 25C), TACS streptavidin-HRP conjugate (1:1600 dilution for 20 min), and TMB substrate solution
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Recent studies have demonstrated that vascular calcification as an active and tightly regulated pathological process, involving VSMC phenotypic transformation, resembling osteogenesis [92, 103, 104]
Beck JD, Andreeff M , Haghbin M, Miller D, Good RA, Gupta S
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