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Islet isolation After mice were killed by CO 2 asphyxiation, the pancreas was inflated via the pancreatic duct with collagenase type V (0.7 mg/ml in HBSS), excised and digested at 37C for 10 to 15 min

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These typical meals are usually the same size, and served around the same time each day

Evidence Signals and What They Cannot Prove Yet Early research can show whether a medicine looks promising, but it cannot answer every practical question

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