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The inner chamber was filled with 200 l M199, and the outer chamber was filled with 600 l M199 containing 1% FBS and 1 g/ml BPC 157

The recommended daily dose of vitamin B12 for adults is 2.4 micrograms, but higher doses have been shown to be safe

Brand-Miller, J

Aliquoting Protocol Decide on a volume per aliquot based on your typical experiment size (e.g., 50100 L per aliquot) Use low-binding microcentrifuge tubes (protein low-bind or equivalent) Label each tube: compound name, concentration, date, lot number Flash freeze aliquots in liquid nitrogen or dry ice/ethanol bath before transferring to -80C storage Discard any aliquot that has been thawed and refrozen more than once See: Optimal Storage and Stability Guidelines for IGF-1 LR3 Lyophilized Research Peptide for detailed post-reconstitution storage guidance

The symptoms of a B12 deficiency are not necessarily any different between males or females