Unlike Ipamorelin, which is highly selective, GHRP-2 is known in research literature to induce a mild increase in ACTH (cortisol) and prolactin levels, making it a valuable reagent for studying the hypothalamic-pituitary-adrenal (HPA) axis alongside somatotropic pathways
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Islets were washed twice in RPMI prior to preincubation in Krebs-Ringer buffer (KRB) containing 2 mg/ml BSA (S6003, SigmaAldrich) and 3 mM glucose for 1 h at 37C
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