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Overall, the results are inconclusive due to several factors

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Next, the pellet was resuspended in 300 L lysis buffer 3 (10 mmol/L Tris, pH 8.0, 100 mmol/L NaCl, 1 mmol/L EDTA, 0.5 mmol/L EGTA, 0.1% DOC, and 0.5% N -lauroylsarcosine with 0.1 mmol/L PMSF and the EDTA-free protease inhibitor cocktail)