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The protocol was preregistered, searching was comprehensive, study selection and data extraction were independently performed, and evidence certainty was formally assessed using GRADE

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Subsequent in vivo evaluation confirmed that the MSC culture conditions that induced high levels of cellular GSH elicited a remarkable regeneration capacity of hES-MSCs at the chondral defect site, suggesting that combining the selection of appropriate cell culture conditions and the FreSHtracer cell sorting method could further potentiate cartilage repair in the chondral defect model

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