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doi:10.3390/pharmaceutics17030385 2 Yin N, et al

Relationship between the morphological evaluation of the pituitary and the growth hormone (GH) response to GH-releasing hormone Plus arginine in children and adults with congenital hypopituitarism

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To assess the GSSG fraction, first, thiol groups of GSH are blocked with N-ethylmaleimide (NEM) in a subsample, followed by removal of excess NEM with toluene prior to reduction of GSSG to GSH with DTT and derivatization via mbrB (Kranner 1998